Journal: The Journal of clinical endocrinology and metabolism

Article Title: Expression of corticotropin-releasing hormone and its R1 receptor in human endometrial stromal cells.

doi: 10.1210/jcem.82.5.3923

Figure Lengend Snippet: FIG. 2. Restriction enzyme analysis of human CRH DNA fragments obtained from endometrial stromal cells in proliferative and secretory phase (lanes 1 and 3). Lanes 1 and 3 show the undigested PCR products, while lanes 2 and 4 show the correspondent PCR products after PstI digestion. Size marker is on the left side of the gel.

Article Snippet: The membrane was prehybridized in 6 3 SSC, 0.5% SDS, 5 3 Denhardt’s, 0.01 mol/L Na phosphate, 1 mmol/L EDTA, and 100 mg/mL salmon sperm DNA for 2 h at 50 C. An oligoprobe complementary to nucleotides 506–530 of the human CRH gene (16) was 59 end-labeled using T4 polynucleotide kinase (New England Biolabs, Milano, Italy) and g32P ATP (Amersham Life Sciences, Milano, Italy).

Techniques: Marker

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Expression of corticotropin-releasing hormone and its R1 receptor in human endometrial stromal cells.

doi: 10.1210/jcem.82.5.3923

Figure Lengend Snippet: FIG. 5. Reverse HPLC characterization of ir-CRH extracted from hu- man endometrial stromal cell culture medium. The amount of ir-CRH in individual fractions was determined by RIA as described in Ma- terials and Methods. The arrow indicates the fractions where syn- thetic hCRH was eluted from the column.

Article Snippet: The membrane was prehybridized in 6 3 SSC, 0.5% SDS, 5 3 Denhardt’s, 0.01 mol/L Na phosphate, 1 mmol/L EDTA, and 100 mg/mL salmon sperm DNA for 2 h at 50 C. An oligoprobe complementary to nucleotides 506–530 of the human CRH gene (16) was 59 end-labeled using T4 polynucleotide kinase (New England Biolabs, Milano, Italy) and g32P ATP (Amersham Life Sciences, Milano, Italy).

Techniques: Cell Culture